Engineering of Self-Assembled Fibronectin Matrix Protein and Its Effects on Mesenchymal Stem Cells

Fibronectin (FN) contributes to cell adhesion, proliferation, and differentiation in various cell types. To enhance the activity of fibronectin at the sites of focal adhesion, we engineered a novel recombinant fibronectin (FNIII10) fragment connected to the peptide amphiphile sequence (PA), LLLLLLCCCGGDS. In this study, the effects of FNIII10-PA on rat mesenchymal stem cells (rMSCs) were compared with those of FNIII10. FNIII10-PA showed the prominent protein adhesion activity. In addition, FNIII10-PA showed a significantly higher effect on adhesion, proliferation, and differentiation of rMSCs than FNIII10. Taken together, the FNIII10-containing self-assembled sequence enhanced rMSCs adhesion, proliferation, and differentiation.     

基于LabVIEW的软管脉冲实验模糊PID控制系统研究(英文)

软管脉冲实验台一般使用电液伺服系统,占用空间小,原理结构简单,信号跟踪能力强,在工业控制领域应用广泛。然而,由于液压反应速度慢、干扰因素多,因而控制精度低,系统不稳定;而传统的液压脉冲实验台单纯PID的控制方式原理简单,但参数调节困难。本文针对控制系统的特殊要求,提出了一种模糊PID控制方法,既保留了原控制系统的优点,又改善了原系统存在的参数时变、不稳定、滞后等缺点,提高了系统的适应能力和精度。经反复调试和试验,结果表明,该系统有效地解决了系统不稳定、滞后严重和参数时变等问题。     

溴化锂水溶液常压下沸腾再生实验研究

针对常规除湿溶液Li Br水溶液,在常压条件下,展开沸腾再生过程实验研究。根据实验需求确定了正交实验方案,实验因素包括溶液初始浓度、初始温度、热流密度、冷却水流量,实验结果表明,各因素对于常压下沸腾再生速率的影响程度依次为热流密度、初始温度、初始浓度、冷却水流量。其中热流密度和初始温度对再生速率影响显著。     

Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.     

中空碳基材料在电解水中的研究进展

氢能所具有的清洁、高能量密度特点，使其成为一种未来的理想能源。相较于石油、天然气等的热解制氢技术，利用可再生清洁能源进行电催化分解水制氢具有高效和清洁无污染的特点，且获得氢气产物纯度高，具备大规模发展的潜力。而在大规模水电解过程中，电催化剂是不可或缺的元素之一。它能有效地加速电解水在阴阳两极反应的动力学过程。传统的贵金属基催化剂具有良好的电催化析氢、析氧活性，但成本高昂、储量稀缺，从而限制了其规模化地推广及应用。开发新型高效廉价的非贵金属基电催化剂已成为时下研究热点。中空碳基纳米材料集成了中空材料和碳基材料的优势，作为电催化剂，在电解水方面有着潜在的应用价值。本文总结了近年来微纳米结构碳基中空材料作为新型电解水催化剂的研究进展，介绍了高效碳基中空析氧/析氢催化剂的设计原则和相应的设计策略，并对开发持久高效的中空碳基电解水催化剂进行了总结和展望。     

Kinetics of Soybean Oil Hydrolysis on Niobium Catalysts

The catalytic hydrolysis of soybean oil was used as an alternative for the production of monoglycerides (MG) and diglycerides (DG). The reactions were conducted in a stainless-steel tubular reactor in the temperature range of 240–290 °C, on niobium phosphate (NBP) and niobium oxide (NBO) as catalysts. In the hydrolysis reactions at 270 °C, the maximum selectivities of the products of interest were obtained at 22 % MG and 48 % DG for the reaction with NBP, and 7 % MG and 33 % DG with NBO, for 59 % and 36 % of triglyceride conversion in 10 min, respectively. The proposed kinetic model presented a good fit of the theoretical model with the experimental data, showing that the previous hypotheses considered for the mechanism development are suitable for describing the kinetics of soybean oil hydrolysis.     

砖混再生粗骨料混凝土力学性能及工程应用研究

采用城市砖混结构拆除建筑垃圾加工制成的再生粗骨料,用不同配合比设计方法制备了砖混再生粗骨料混凝土,研究了砖混再生粗骨料对混凝土工作性、抗压强度、弹性模量、峰值应力、峰值应变以及应力应变关系的影响.结果 表明,相比较天然骨料和废弃混凝土制备的再生骨料,砖混再生骨料空隙率大,吸水率大,压碎指标高.使用附加用水量配合比方法制备的砖混再生粗骨料混凝土在力学性能上低于标准配合比方法制备的砖混再生粗骨料混凝土,但工作性能较好.随着砖混再生粗骨料取代率的增加,再生混凝土的力学性能呈现不同程度的降低.当取代率为100％时,使用标准配合比和附加用水量方法制备的再生混凝土28 d抗压强度分别降低了26.1％和11.9％,弹性模量分别降低了23.9％和22.9％,峰值应力分别降低了13.0％和21.9％,峰值应变分别增大了25.3％和14.9％.工程实践表明,采用砖混再生粗骨料配制的再生混凝土可以满足中低强度等级泵送混凝土的性能要求.     

运行压力对超声雾化溶液除湿系统性能的影响

基于溶液除湿热质传递过程中所遵循的质量平衡、能量平衡模型，以超声雾化溶液除湿系统（UADS）为例，探讨了在不同空气处理温度、湿度下，系统运行压力对除湿性能的影响。结果表明：随着运行压力的升高，UADS系统的除湿性能显著改善。特别是在所处理空气入口温度较高时，提升系统运行压力对除湿效率的提高作用更加显著。同时，当所处理空气的含湿量较大时，提高运行压力对系统除湿速率的增幅明显，而对除湿效率的改善作用减弱。此外，当除湿系统在较高压力下运行时，其除湿效率受所处理空气入口温湿度变化而产生的波动减小，系统性能稳定性显著改善。所得结果可对溶液除湿系统的高效、稳定运行提供积极参考。